Antibody Characterization and Sample Preparation

During antibody characterization, labs will need to validate lot-to-lot reproducibility. Or, every lot they are manufacturing, the environment has been controlled and every lot is behaving as previously defined

The analytical lab is involved in many steps of lead optimization, including antibody characterization and then in every step of scale-up. When looking at characterization, the lab looks at the purity, potency, and identity of the protein.

Purity – Dosing will depend on the level of glycosylation. Are there breakdown products? Are there contaminants?
Potency- Should it be glycosylated? Does each batch contain the right combination of structures?
Identity – Does the protein have the right size, right charge, and glycosylation? 

During scale-up, manufacturing and process development are critical in validating that the antibody is behaving correctly in children and grandchildren generations. Factors such as temperature, pH, and media additives can impact cell viability and the bi-product: proteins. Each lot needs characterized to ensure critical quality attributes have been validated. 

The below resources are tools to help you characterize the binding site of your antibody to its target protein. 

Products for Antibody Characterization and Sample Prep

Optima AUC 分析型超速离心机

Analytical Ultracentrifuge

 Our highest throughput centrifuge. Spin through the analytical ultracentrifuge to achieve characterization of the protein's properties. 
Biomek i-Series自动化工作站

iSeries Automated Liquid Handlers

Automate protein characterization assays with tools such as solid phase extraction and protein digest. Automation can reduce sources of error associated with manual pipetting and improve the reliability of characterization assays. 

CytoFLEX 流式细胞仪

Research Flow Cytometers

 Identify and characterize surface markers using flow cytometry. 

Research Flow Reagents

 Highlight your antibody of interest with our flow reagents. 

Helpful Links

Content and Resources

Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series Protein digestion and analysis by mass spectrometry is an essential process in numerous proteomic approaches such as biomarker discovery. However, the sample preparation can be tedious and error prone. Automation can relieve the burden of sample preparation while also reducing the variability between users and across experiments.
Flexible ELISA automation with the Biomek i5 Workstation Enzyme-linked immunosorbent assays (ELISAs) are a common analytical tool for detecting the presence of a protein or other molecules of interest. While the workflow is broadly conserved, numerous reagent addition and wash steps make this a labor-intensive endeavor. Automation of these steps not only relieves this labor burden but can lead to improvements in result consistency.
Hydraulic Particle Counter Sample Preparation Best Practices for repeatable particle count results
10 Things to Consider When Exploring Automation for ELISA With limited resources, multiple improvements to implement, other demands that require your attention, and automation solutions that all sound the same, you might be enticed to let your budget lead the way!