小孔管

小孔管是一个空心玻璃管,其上有一个孔,通过它,细胞被吸进库尔特计数器。该管充满盐溶液,电极放置在管的内部和外部,电流流经管中的孔。测量样品时,在管子内部放置一个真空,将细胞或颗粒拉过孔。每次细胞进入孔径孔时,它都会取代盐溶液,从而产生可见的电脉冲。脉冲的大小告诉您细胞(或任何粒子)穿过孔时的大小。脉冲的数量告诉你有多少东西穿过了这个洞。aperture tubes for multisizer

用于 Multisizer 4e 的库尔特小孔管

Aperture
(Nominal
Diameter, μm)
Particle Diameter Range (μm) Particle Volume Range (μm3or fL)
Total Lower
Limit
Upper
Standard
Limit
Upper
Extended
Limit
Total Lower
Limit
Upper
Standard
Limit
Upper
Extended
Limit
Part
Number
10† 0.2 - 8.0 0.2μm 6.0μm 8.0μm 0.004 - 268 x 103μm3 0.004μm3 113μm3 268μm3 B42812
20† 0.4 - 16 0.4μm 12μm 16μm 0.034 - 2.14 x 103μm3 0.034μm3 905μm3 2,144μm3 A36390
30† 0.6 - 24 0.6μm 18μm 24μm 0.113 - 7.24 x 103μm3 0.113μm3 3,053μm3 7,238μm3 A36391
50†† 1.0 - 40 1.0μm 30μm 40μm 0.524 - 33.5 x 103μm3 0.524μm3 14,137μm3 33,510μm3 A36392
70†† 1.4 - 56 1.4μm 42μm 56μm 1.44 - 92.0 x 103μm3 1.44μm3 38,792μm3 91,952μm3 A36393
100†† 2.0 - 80 2.0μm 60μm 80μm 4.19 - 268 x 103μm3 4.19μm3 113 x 103μm3 268 x 103μm3 A36394
140†† 2.8 - 112 2.8μm 84μm 112μm 11.5 - 736 x 103μm3 11.5μm3 310 x 103μm3 736 x 103μm3 A36395
200†† 4.0 - 160 4.0μm 120μm 160μm 33.5 - 2.14 x 106μm3 33.5μm3 905 x 103μm3 2.14 x 106μm3 A36396
280 5.6 - 224 5.6μm 168μm 224μm 92.0 - 5.88 x 106μm3 92.0μm3 2.48 x 106μm3 5.88 x 106μm3 A36397
400 8.0 - 320 8.0μm 240μm 320μm 268 - 17.2 x 106μm3 268μm3 7.24 x 106μm3 17.2 x 106μm3 A36398
560 11.2 - 448 11.2μm 336μm 448μm 736 - 47.1 x 106μm3 736μm3 19.9 x 106μm3 47.1 x 106μm3 A36399
800††† 16 - 640 16μm 480μm 640μm 2145 - 137 x 106μm3 2145μm3 57.9 x 106μm3 137 x 106μm3 A40889
1000††† 20 - 800 20μm 600μm 800μm 4189 - 268 x 106μm3 4189μm3 113 x 106μm3 268 x 106μm3 A36400
2000††† 40 - 1600 40μm 1200μm 1600μm 33,510-2140 x 106μm3 33,510μm3 905 x 106μm3 2140 x 106μm3 A36401

High resolution tubes have an aperture thickness 120% greater than normal apertures thus, extending the time the particle takes to pass through the aperture, giving greater discrimination of particle size.

Hi-Res 50 1.0 - 40 1.0μm 30μm 40μm 0.524 –33.5x 103μm3 0.524μm3 14,137μm3 33,510 μm3 A44581
Hi-Res 70 1.4 – 56 1.4μm 42μm 56μm 1.44 –92.0x 103μm3 1.44μm3 38,792 μm3 91,952 μm3 A44582
Hi-Res 100 2.0-80 2.0μm 60μm 80μm 4.19 –268x 103μm3 4.19μm3 113x 103μm3 268x 103μm3 A44583
Hi-Res 140 2.8 – 112 2.8μm 84μm 112μm 11.5 –736x 103μm3 11.5μm3 310x 103μm3 736x 103μm3 A44584
Hi-Res 200 4.0 – 160 4.0μm 120μm 160μm 33.5 –2.14 x 106μm3 33.5μm3 905x 103μm3 2.14 x 106μm3 A44585
s † 范围取决于系统清洁度和环境电磁噪声
†† 提供高分辨率孔管
††† 范围取决于样本密度

孔管范围
总范围:孔径直径的2%至80%
标准范围:孔径直径的2%至60%
扩展范围:孔径直径的 60% 到 80%

孔径动态范围
标准 1:30(直径),扩展 1:40(直径)
标准 1:27,000(按容量),扩展 1:64,000(按容量)

库尔特仪器的微球校准标准

Part Number Name Nominal Size Primary Aperture(s) for calibration* Other Apertures for verification**
6602792 STANDARD 2 µm, LATEX PARTICLE, NIST TRACEABLE 2µm 10 µm 20 µm, 30 µm
6602793 STANDARD 3 µm, LATEX PARTICLE, NIST TRACEABLE 3 µm 20 µm 10 µm, 30 µm
6602794 STANDARD 5 µm, LATEX PARTICLE, NIST TRACEABLE 5 µm 30 µm 10 µm, 20 µm, 50 µm
6602796 STANDARD 10 µm, LATEX PARTICLE, NIST TRACEABLE 10 µm 50 µm, 70 µm 20 µm, 30 µm, 50 µm, 70 µm, 100 µm, 140 µm, 200 µm
6602797 STANDARD 15 µm, LATEX PARTICLE, NIST TRACEABLE 15 µm 70 µm 50 µm, 100 µm, 200 µm, 280 µm
6602798 STANDARD 20 µm, LATEX PARTICLE, NIST TRACEABLE 20 µm 100 µm, 140 µm 140 µm, 200 µm, 280 µm, 400 µm
6602799 STANDARD 30 µm, LATEX PARTICLE, NIST TRACEABLE 30 µm 200 µm 100 µm, 140 µm, 200 µm, 280 µm, 400 µm, 560 µm
6602800 STANDARD 43 µm, LATEX PARTICLE, NIST TRACEABLE 43 µm 280 µm 2800 µm, 400 µm
6602802 STANDARD 90 µm, LATEX PARTICLE, NIST TRACEABLE 90 µm 400 µm, 560 µm 1000 µm, 2000 µm
8321515 STANDARD 200 µm, LATEX PARTICLE, NIST TRACEABLE 200 µm 1000 µm 560 µm, 2000 µm
383601 STANDARD 300 µm, LATEX PARTICLE, NIST TRACEABLE 300 µm 2000 µm 560 µm, 1000 µm

*使用推荐带主孔管的微珠进行孔管校准。
**校准后,可以使用其他尺寸的微珠来验证孔径的性能。
建议使用孔径尺寸范围为 7-30% 的微珠校准仪器。

选择正确的仪器用于细胞计数和颗粒表征

 

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