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FAQ on Centrifuge Rotors
What are the main types of centrifuge rotors?
The main types of centrifuge rotors include fixed-angle, swinging-bucket, vertical, near-vertical, zonal, continuous flow, analytical ultracentrifuge, and elutriation rotors. Each rotor design is optimized for specific separation methods, sample volumes, and performance requirements.
What is a continuous flow rotor used for?
Continuous flow rotors are designed for processing large sample volumes without repeatedly stopping the centrifuge. They are commonly used for harvesting cells, viruses, organelles, and biological products in research and bioprocessing applications.
What is the difference between a fixed-angle rotor and a swinging-bucket rotor?
Fixed-angle rotors hold tubes at a constant angle and are commonly used for rapid pelleting applications. Swinging-bucket rotors allow tubes to swing into a horizontal position during centrifugation, making them well suited for density-gradient separations, improved sample layering, and higher-resolution fractionation.
When should a vertical rotor be used in centrifugation?
Vertical rotors are typically used for isopycnic and density-gradient separations in ultracentrifugation workflows. Their design minimizes particle travel distance, helping reduce run times while maintaining efficient separation performance.
How do I choose the right centrifuge rotor for my application?
Choosing the right centrifuge rotor depends on factors such as sample volume, particle size, required throughput, centrifugation method, and desired separation outcome. Fixed-angle rotors are often preferred for pelleting, while swinging-bucket, vertical, and continuous flow rotors are selected for specialized separation and large-volume processing workflows.