贝克曼Vi-CELL BLU 细胞计数和活率分析仪

What are viable cells?

Viable cells are live, healthy cells in a sample. In cell viability assays, viable cells are typically assessed by indicators such as intact cell membranes, cellular function, enzymatic activity, or metabolic activity. In the context of trypan blue, viable cells are live cells with intact membranes and sufficient cellular health to support downstream culture, analysis, or processing decisions. Cell viability measurements help researchers understand the quality and health of a cell population before moving forward with experiments or bioprocessing steps.

Can I compare results with historical data with the Vi-CELL BLU 2.0 software?

Yes. The Vi-CELL BLU 2.0 software is designed to support a seamless transition with improved alignment to historical data, helping laboratories maintain data comparability while benefiting from enhanced accuracy and performance.

How does trypan blue work?

Trypan blue works through a dye-exclusion principle. Viable cells have intact cell membranes that exclude the dye, so they remain unstained, while non-viable cells have compromised membranes that allow trypan blue to enter, making them appear blue. This makes trypan blue a widely used method for distinguishing live cells from dead cells during cell counting and viability analysis.

The Vi-CELL BLU Cell Viability Analyzer automates the trypan blue dye exclusion method, helping reduce manual microscopy, pipetting, and hemocytometer-based workflows.

Does the Vi-CELL BLU Cell Viability Analyzer support regulatory compliance?

Yes. The system includes data integrity and compliance features such as audit trails, electronic signatures, secure user sign-on, user-level permissions, and tools that support 21 CFR Part 11 compliance requirements.

Can the Vi-CELL BLU Cell Viability Analyzer support high-throughput workflows?

Yes. The system offers flexible sample introduction through a 24-position carousel or a 96-well plate format, making it suitable for both routine laboratory workflows and higher-throughput applications. It also supports integration with automation platforms.

Does the Vi-CELL BLU Cell Viability Analyzer integrate with automated bioprocessing systems?

Yes. The Vi-CELL BLU Cell Viability Analyzer can be integrated with automation and bioprocessing platforms, including Sartorius Ambr® systems, Biomek automated workstations, and the Flownamics Seg-Flow S3 automated sampling system, helping streamline cell culture workflows.