细胞系开发

细胞系开发是选用细胞机器制造治疗性生物制剂或其他目标蛋白的过程。下文列举了适用于这一关键过程的一系列产品。


用于细胞系开发的产品

Optima 系列超速离心机

 性能卓越的超速离心机系列，助您实现样品的快速、高效分离
valitatiter igg quantification assay kit val003

ValitaTiter 检测

 简单、快速的 IgG 定量分析方法

CytoFLEX SRT 流式分选仪

 CytoFLEX SRT 流式细胞分选仪助您轻松简化复杂的分选任务
Biomek i-Series自动化工作站

Biomek i-Series 自动化工作站

 基于吸头的自动化液体处理解决方案

Echo 525声波移液系统

 无吸头、全自动、非接触式液体处理解决方案
Avanti JXN-26 高速冷冻离心机

Avanti JXN 系列高效离心机

 专为提供更多的实验通量，样品周转以及更大的工作流程实验控制所设计。
Vi-CELL MetaFLEX 高速细胞培养生化分析仪

Vi-CELL MetaFLEX

 速度快，可靠性高，样品量小

Vi-CELL BLU细胞计数及活率分析仪

 自动化了用于细胞计数和活率分析的细胞混匀和台盼蓝染色步骤。

Avanti J-15R 系列高速冷冻离心机

 功能齐全同时配备卓越的离心技术

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Content and Resources

Efficient Factorial Optimization of Transfection Conditions Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor. However, whether one is transfecting plasmid DNA or inhibitory RNAs, different cell lines can have significant differences in the ease of transfection or cellular survival. Determining the optimal plating conditions for high transfection efficiency and low cytotoxicity can be highly involved.
Automating Cell Line Development for Biologics Cell line development is a highly-involved, multi-week workflow that involves the plating, screening, and expansion of single cell-derived clones to establish a protein-expression cell line. This complex process required significant throughput of sterile cell manipulations, long-term incubations, centrifugation, cell counting, monoclonality determination and growth tracking, and protein production assessment.
Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR This application note details how you can use the plate loader feature on the Vi-CELL BLU to match cell types to give cell counts and viability levels equivalent to those obtained on existing Vi-CELL XR instruments.
Considerations of Cell Counting Analysis when using Different Types of Cells This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
Cell Line Development – Limiting Dilution Limiting dilution is a traditional approach to achieve monoclonality. It entails diluting cells to the level that there is one cell on average per unit of volume plated in the wells. However, as predicted by the Poisson distribution, roughly one third of the plated wells will have zero cells, one third of wells will have a single cell, and one third will have multiple cells per well (Coller and Coller 1986).