纳米颗粒研究

Leverage the power of flow cytometry for the detection and characterization of cellular origin nanoparticles

Interest in nanoparticles of cellular origin is exploding due to new insights into the role these particles play in extracellular communication processes and the potential for detecting pathological states via liquid biopsy.

Several fundamental capabilities of flow cytometry make this an attractive platform for extracellular vesicle research, such as the ability to detect large numbers of events and discriminate rare events while collecting information on surface composition.

Recent developments in laser and electronics design pushed the boundaries of reliably detecting events in the nanoparticle size range. The CytoFLEX Flow Cytometer is the first benchtop instrument with acceptable noise-to-signal discrimination for the reliable detection of particles down to 80 nm. 

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Tech Note: Violet Side Scatter Use in Nanoparticle Detection How to Use Violet Side Scatter to Detect Nanoparticles on the CytoFLEX Flow Cytometer
Nano- and Microparticle Bead Mix for Use as a Sizing Standard in Flow Cytometry The CytoFLEX platform is distinguished for not only its fluorescence sensitivity but also its ability to detect in the nanoparticle range using Violet Side Scatter. This poster, presented at Cyto in 2016 describes a NIST-traceable bead mix for use in standardizing flow cytometry experiments.
Resolving Dyes with Identical Emission Wavelengths on the CytoFLEX Compensation in flow cytometry is a special case of spectral deconvolution. In this poster we demonstrate that the CytoFLEX can deconvolute highly overlapping fluorophores using the compensation algorithm.
A Prototype CytoFLEX for High-Sensitivity, Multiparametric Nanoparticle Analysis In this poster, we will demonstrate the VSSC-based size resolution and fluorescence sensitivity of our prototype using a variety of NIST-traceable size standards and fluorescent nanoparticles
Preparing a CytoFLEX for Nanoscale Flow Cytometry In this poster, we will demonstrate how to properly setup and clean a CytoFLEX flow cytometer for NP analyses

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