OptiMATE 全自动密度梯度制备系统
专为优化密度梯度超速离心工作流而设计
OptiMATE 全自动密度梯度制备系统凭借自动化梯度液灌注与密封功能，可自动完成包括梯度制备、精准梯度液灌注和超离管密封等所有离心前步骤，将传统手工操作中繁琐且易出错的 密度梯度超速离心（DGUC）步骤，简化为易于维护的自动化工作流，显著缩短运行时间，并确保梯度构建的纯度或产量。
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OptiMATE 全自动密度梯度制备系统的优势功能
自动化梯度液灌注与密封
- 显著减少错误并提高可重复性
- 适配常见的贝克曼库尔特样品管尺寸
- 适配 Quick-Seal快封管, OptiSeal指封管，和开口管
预成型线性梯度
- 轻松生成线性梯度和阶梯梯度
- 显著缩短线性梯度构建的运行时间（缩减超 75%）
智能化操作界面搭配图形化方法创建
- 新操作员培训仅需几小时
- 标准化方法确保不同批次及操作员间实验结果的一致性
- 实时梯度曲线可视化掌控
自动化梯度液灌注与密封
预成型线性梯度
智能化操作界面搭配图形化方法创建
- 全自动分液与封管，轻松实现线性梯度与阶梯梯度构建
- 预成型线性梯度，显著缩短实验运行时间
- 全自动工作流，确保一致性，减少人工移液错误
- 无需专业经验，一键快速生成高质量梯度
- 广泛适用于 氯化铯 (CsCl), 碘克沙醇 (IDX)和蔗糖等多种介质
Although gradient profiles indicate sufficient separation even at 4 hours, control pre-formed experiments (left) were run for 5 hours while AAV pre-formed experiments (right) were run for 5 hours to improve band resolution/sharpness.
相关产品或零部件
A-100/18 Fixed-Angle Rotor, 6 x 175 µL, 95,000 rpm, 149,000 x g
A-100/18 Rotor is a fixed-angle rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges, made of aluminum and anodized silver. Major applications: Fast pelleting of very small samples.
A-95 Fixed-Angle Rotor, 4 x 450 µL, 95,000 rpm, 178,000 x g
The A-95 Rotor is a fixed-angle rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Major applications: Fast pelleting of very small samples.
A-100/30 Fixed-Angle Rotor, 6 x 240 µL, 92,000 rpm, 167,000 x g
A-100/30 Rotor is a fixed-angle rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Major applications: Fast pelleting of very small samples.
ACR-90 Chylomicron Rotor, 3.5 mL, 90,000 rpm, 122,000 x g
The ACR-90 Chylomicron Rotor is a specialty rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Major applications: Rapid clarification of lipemic blood samples. Consists of a supporting base, a disposable polyethylene liner, a stainless steel lid, adapters for 2.4 mL liners, and rotor stand/vise.
Aluminum Batch Rotor 7 mL, 90,000 rpm, 132,000 x g
Batch rotor, aluminum, for 2 mL to 7 mL volumes. For use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Sample is placed in the rotor body istelf or in one of two types of polyethylene liners. A plastic window disk is placed between the rotor and the lid to contain samples during centrifugation. Major applications: Rapid processing of fruit paste. Includes rotor stand/vise, o-ring, and bushing.
EM-90 Electron Microscopy Rotor 600 µL, 90,000 rpm, 118,000 x g
EM-90 Electron Microscopy Particle Counting Rotor, Silver Major applications: Sedimenting particles onto filter membranes, glass cover slides, and micropscope grids. For use in Airfuge; Air-Driven Micro-Ultracentrifuges. Includes: 5 mm Sector Core with 600; L capacity (100L per cell) (Part Number 347836), 3 mm Sector Core with 360; L capacity (60; L per cell) (Part Number 347838) Rotor Stand/Vise (Part Number 341252) Rotor Gaskets (Part Number 347840) Sector Core Removal Bolt Template; O-Ring (Part Number 853156) Bushing (Part Number 343197)
相关产品或零部件
A-100/18 Fixed-Angle Rotor, 6 x 175 µL, 95,000 rpm, 149,000 x g
A-100/18 Rotor is a fixed-angle rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges, made of aluminum and anodized silver. Major applications: Fast pelleting of very small samples.
A-95 Fixed-Angle Rotor, 4 x 450 µL, 95,000 rpm, 178,000 x g
The A-95 Rotor is a fixed-angle rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Major applications: Fast pelleting of very small samples.
A-100/30 Fixed-Angle Rotor, 6 x 240 µL, 92,000 rpm, 167,000 x g
A-100/30 Rotor is a fixed-angle rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Major applications: Fast pelleting of very small samples.
ACR-90 Chylomicron Rotor, 3.5 mL, 90,000 rpm, 122,000 x g
The ACR-90 Chylomicron Rotor is a specialty rotor for use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Major applications: Rapid clarification of lipemic blood samples. Consists of a supporting base, a disposable polyethylene liner, a stainless steel lid, adapters for 2.4 mL liners, and rotor stand/vise.
Aluminum Batch Rotor 7 mL, 90,000 rpm, 132,000 x g
Batch rotor, aluminum, for 2 mL to 7 mL volumes. For use in Beckman Coulter Airfuge Air-Driven Micro-Ultracentrifuges. Sample is placed in the rotor body istelf or in one of two types of polyethylene liners. A plastic window disk is placed between the rotor and the lid to contain samples during centrifugation. Major applications: Rapid processing of fruit paste. Includes rotor stand/vise, o-ring, and bushing.
EM-90 Electron Microscopy Rotor 600 µL, 90,000 rpm, 118,000 x g
EM-90 Electron Microscopy Particle Counting Rotor, Silver Major applications: Sedimenting particles onto filter membranes, glass cover slides, and micropscope grids. For use in Airfuge; Air-Driven Micro-Ultracentrifuges. Includes: 5 mm Sector Core with 600; L capacity (100L per cell) (Part Number 347836), 3 mm Sector Core with 360; L capacity (60; L per cell) (Part Number 347838) Rotor Stand/Vise (Part Number 341252) Rotor Gaskets (Part Number 347840) Sector Core Removal Bolt Template; O-Ring (Part Number 853156) Bushing (Part Number 343197)
选择 OptiMATE Gradient Maker 型号
常见问题解答
- 氯化铯
- 碘克沙醇 (OptiPrep™)
- 蔗糖
Sizes
- 16 mm (8.9 to 13.5 mL)
- 25 mm (29.9 to 39 mL)
- 38 mm (94 mL)
- Quick-Seal快封管
- Opti-Seal指封管
- Open-Top开口管
- 聚丙烯（PP）
- 超净管
否，OptiMATE 系统仅适配贝克曼库尔特生命科学公司提供的超速离心管。
可以，这些管路已经过了三次运行测试，可以重复使用。
可以，管路套件可通过高压灭菌器或环氧乙烷（EtO）方式进行灭菌处理。
密度梯度超速离心（DGUC）是一种基于离心的技术，可在基因治疗生产工作流程中对病毒载体（例如，从部分包装和空衣壳中分离出完整的 AAV 颗粒）以及其他材料（如质粒 DNA）进行出色的纯化。DGUC 可根据原始样品中材料的密度形成分层梯度。
- 病毒/病毒载体（例如 AAV）
- 病毒样颗粒和噬菌体
- 细胞外囊泡（EV）
- 蛋白质/蛋白质复合物
- 脂质纳米颗粒
- 合成纳米颗粒或其他纳米材料
- 核酸/质粒
- 脂质分离
技术文件
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