OptiMATE 全自动密度梯度分液系统

专为优化密度梯度超速离心工作流而设计

OptiMATE 全自动密度梯度分液系统凭借自动化梯度液灌注与密封功能，可自动完成包括梯度制备、精准梯度液灌注和超离管密封等所有离心前步骤，将传统手工操作中繁琐且易出错的 密度梯度超速离心（DGUC）步骤，简化为易于维护的自动化工作流，显著缩短运行时间，并确保梯度构建的纯度或产量。

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资源 + 工具
下载彩页 技术文档 Density Gradient Media OptiMATE Density Gradient Calculator OptiXTRACT Sample Recovery Aid
OptiMATE gradient maker for density gradient centrifugation

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OptiMATE 全自动密度梯度制备系统的优势功能

自动化梯度液灌注与密封

预成型线性梯度

  • 轻松生成线性梯度和阶梯梯度
  • 显著缩短线性梯度构建的运行时间（缩减超 75%）
    •  

智能化操作界面搭配图形化方法创建

  • 新操作员培训仅需几小时
  • 标准化方法确保不同批次及操作员间实验结果的一致性
  • 实时梯度曲线可视化掌控

OptiMATE 全自动密度梯度制备系统简介

 


自动化梯度液灌注与密封

预成型线性梯度

智能化操作界面搭配图形化方法创建

自动化、一致且高效的梯度液灌注


  • 全自动分液与封管，轻松实现线性梯度与阶梯梯度构建

  • 预成型线性梯度，显著缩短实验运行时间

  • 全自动工作流，确保一致性，减少人工移液错误

  • 无需专业经验，一键快速生成高质量梯度

  • 广泛适用于 氯化铯 (CsCl), 碘克沙醇 (IDX)和蔗糖等多种介质
OptiMATE Gradient Maker performance data

Although gradient profiles indicate sufficient separation even at 4 hours, control pre-formed experiments (left) were run for 5 hours while AAV pre-formed experiments (right) were run for 5 hours to improve band resolution/sharpness.

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Resources

OptiMATE 全自动密度梯度制备系统助您快速纯化腺相关病毒

 

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OptiMATE 全自动密度梯度制备系统助您减少病毒载体纯化的可变性和手工操作时间

 

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OptiMATE 全自动密度梯度制备系统
的优势功能

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Explore More Solutions for Density Gradient Centrifugation

1
Media preparation

OptiMATE Gradient Media
2
Gradient generation

OptiMATE Gradient Maker
3
Centrifugation

Optima XPN Ultracentrifuge
4
Band extraction

OptiXTRACT Sample Recovery Aid

FAQ下载

技术文件

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FAQ on the OptiMATE Gradient Maker

What tubes are compatible with the OptiMATE Gradient Maker?

Sizes

  • 16 mm (8.9 to 13.5 mL)
  • 25 mm (29.9 to 39 mL)
  • 38 mm (94 mL)
Formats
  • Quick-Seal
  • OptiSeal
  • Open-Top
Materials
  • Polypropylene
  • Ultra-Clear
Filling and sealing performance are impacted by tube material, tube size, and tube volume. Not all tubes meeting the above criteria may be suitable. For a complete list of compatible rotors and tubes, please see the instrument brochure and instructions for use (IFU).

What density gradient reagents are compatible with the OptiMATE Gradient maker?

  • Cesium Chloride
  • Iodixanol (OptiPrep™)
  • Sucrose

Are the tubing lines reusable?

Yes, tubing lines are reusable. They have been tested to three runs.

Can I use 3rd-party tubes with the OptiMATE Gradient Maker?

No, OptiMATE is only compatible with ultracentrifuge tubes provided by Beckman Coulter Life Sciences.

What is density gradient centrifugation?

Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty capsids), along with other materials (such as plasmid DNA) in gene therapy production workflows. DGUC results in a layered gradient based on the density of materials in the original sample.

What applications is density gradient ultracentrifugation used for?

  • Viruses / viral vectors (e.g., AAV)
  • Virus-like particles and bacteriophage
  • Extracellular vesicles (EVs)
  • Proteins / protein complexes
  • Lipid nanoparticles
  • Synthetic nanoparticles or other nanomaterials
  • Nucleic acids / plasmids
  • Lipemic separations