BioLector XT Microbioreactor

Microfluidic Bioprocess Control

Cultivation conditions

TEMPERATURE
10 – 50 °C (min. temp.: 8 °C below ambient temp.)

SHAKING SPEED
100 – 1500 rpm (3 mm diameter)

ENVIRONMENTAL CONDITIONS
Active humidification
Ambient air
1 – 100 % O2 (optional)
0 – 12 % CO2 (optional)
Anaerobic cultivation (optional)

OXYGEN OPTODES
0 – 100 % dissolved oxygen*1

pH OPTODES
pH 4 – 7.5 (depending on plate)

MTP READING TIME
2.7 min / filter / 48 wells @ 1000 rpm

Microfluidic features*2

TRIGGERED pH CONTROL (CLOSED LOOP CONTROLLER)
pH control range: 4.0 – 7.5 (depending on plate)
Fully editable PI control
Slow, medium and fast PI default settings

FEEDING OPTIONS
Two sided pH control (alkali and acid)
One sided pH control and one feed line (alkali or acid + one feed)
Two feed lines

FEEDING PROFILES
Profile equation: dV/dt =A+B×t+C×eD×t
Constant: A [μL/h]
Linear: A [μL/h] and B [μL/h²]
Exponential: A [μL/h], B [μL/h²], C [μL/h] and D [h-1]
Pulse feed
Full feeding profile flexibility permits broad experimental design

PUMP RATE
Up to 665 pump strokes per hour

System dimensions

System Dimensions

Gassing lid dimensions

Gassing Lid Dimensions

Gassing Lid Dimensions2

Available optional modules

Part no.

 Module description Additional feature
 Note
E-XTMF
 TradeGothicLT-Extended
 Active control of pH according to online signals and continuous feeding of up to two solutions only with Microfluidic plates
E-O2XT-100
 O2 up-regulation module
 Control of gas atmosphere in head space: 21 – 100 % O2
E-O2XT-25
 O2 down-regulation module
 Control of gas atmosphere in head space: 1 – 21 % O2
 use only with N2
E-CO2XT-12
 CO2 up-regulation module
 Control of gas atmosphere in head space: 0 – 12 % CO2
E-AN-300
 Anaerobic cultivation module
 Gassing with 100 % N2 allows cultivation of organisms in anaerobic conditions
 use only with N2
All optional modules compatible in one BioLector microbioreactor device.

Microtiter plates

FLOWERPLATE
48 cultivation wells
Filling volume: 800 – 1900 μL (rpm dependent)
High OTR and high kLa

ROUND WELL PLATE
48 cultivation wells
Filling volume: 1000 – 2400 μL (rpm dependent)
Lower OTR and low shear force

MICROFLUIDIC PLATE
Available as both FlowerPlate and Round Well Plate
32 cultivation wells controlled by 16 reservoir wells
Maximum filling volumes in reservoir wells: 1800 µL (FlowerPlate) and 2000 µL (Round Well Plate)
Same filling volumes for cultivation wells as in 48 well plate

Lab space and material requirements

Flat surface with a minimal loading capacity of 100 kg for BioLector XT microbioreactor or 250 kg for BioLector XT microbioreactor with the valve control unit*2
Device weight: 58 kg for BioLector XT microbioreactor (61 kg with microfluidic module) and 44 kg for valve control unit*2
1x power supply for BioLector XT microbioreactor: 90-264 VAC, 47~63 Hz 1x power supply for laptop: 90-230 VAC, 50/60 Hz 1x power supply for valve control unit*2: 90-264 VAC, 47~63 Hz (US/Canada); 85-264 VAC, 47~63 Hz (EU, ROW)
Microfluidics: requires 4 to 6 bar dry and oil-free compressed air, 6 mm ØOUT push-in connection
Gassing modules (O2 up, O2 down, CO2 up, anaerobic module): require 1.5-2 bar dry and oil-free O2, CO2, or N2; 4 mm ØOUT push-in connection
Humidity control: 400 mL deionized water

 

*1 100 % corresponding to the DO level reached while gassing with 100 % O2 without O2 consumption
*2 only with optional microfluidic module OTR: Oxygen transfer rate [mmol/L/h]
kLa: Volumetric oxygen transfer coefficient [h-1]
m2p and the m2p logo are the trademarks or registered trademarks of m2p-labs GmbH in the US and other countries.
m2p-labs is a Beckman Coulter company.

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