Valita Titer Gen III Assay – the next generation in IgG titer measurement

Precision and efficiency

This cutting-edge product is designed to revolutionize your workflow by enabling the measurement of low concentration titer early in the process. It provides the reliability of standard methods with the added advantages of reduced time and easier handling.

With a focus on ensuring high accuracy and precision, it leaves no doubt in your results. Moreover, it supports workflow flexibility, thanks to its increased signal stability and broad binding properties.

Assay Features 

• New functional range from 0.5 – 100 mg/L


• Excellent correlation (R² < 0.99) with established methods such as HPLC and BLI


• Increased accuracy, with >96% accuracy and <1.2% relative standard deviation (RSD)


• Binding Human IgG 1,2,4 and rabbit IgG, Fc-fusion proteins, and bispecific antibodies 


• Excellent signal stability for more than 50 mins  

Excellent correlation with established methods such as HPLC and BLI, even below 20 mg/L IgG titer

When comparing the Valita Titer Gen III assay with other standard methods to assess IgG titer, such as bio-layer interferometry (BLI) or high-performance liquid chromatography (HPLC) with a protein A column, the resulting data indicated a high correlation of R² > 0.998.  This correlation confirms the high reliability of the Gen III assay when analyzing IgG titer.

Especially in low titer ranges  < 20 mg/L, the Valita Titer Gen III assay shows its high resolution capability with a range of 0.5-100 mg/L. This lets you implement titer quantification early in your clone screening process.

Valita Titer Gen III Assay vs BLI & HPLC Protein A

The Valita Titer Gen III assay showed comparable results when tested with different antibody types, such as human IgG 1, IgG 2, IgG 4, rabbit pAb and bispecific antibody.

Valita Titer Gen III Assay vs BLI (Sub-20mg/L)

Measurement of Fc-Fusion proteins of 76kDa or 150kDa is possible with the Valita Titer Gen III assay and gives robust results.

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Increased accuracy for IgG quantification

This new assay provides high accuracy when measuring IgG titer in your samples. With over 96% accuracy it can predict IgG concentration when you measure samples of known concentration. The low standard deviation and coefficient of variation (2.1%) indicate high precision and low variation between measured sample concentrations.

Valita Titer Gen III: Sample Interpolation

Comparison of IgG concentrations measured with Valita Titer Gen III output results. The bar chart shows the average interpolated concentration from 3 replicates, with error bars representing the standard deviation. Average recovery = 98.9 %, average standard deviation = 0.29 mg/L and average %CV = 2.1 across the entire range. 

Versatile detection from human and rabbit IgG to bispecific and Fc-fusion proteins

The Valita Titer Gen III assay probe can detect and quantify standard IgG from human or rabbit species as well as bispecific antibodies (bsAbs). It ensures robust performance with Fc-fusion proteins across a wide range of molecular sizes.

Valita Titer Gen III Assay - Human, Bispecific and Rabbit IgG

Valita Titer Gen III Assay - Standard IgG vs Fc-Fusion

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Excellent signal stability for more than 50 mins

With a signal stability of up to 55 mins after sample addition, the assay offers exceptional flexibility for automation or batch processing, ensuring consistent results without time pressure.

Valita Titer Gen III Assay - Signal Stability

Samples with a known concentration were measured after 5, 15, 25, 35, 45, and 55 minutes incubation time after sample addition and showed a high correlation.

For Research Use Only. Not for use in diagnostic procedures. Valita, Valita Titer, Valita Aggregation Pure and the ValitaCell logo are the trademarks of ValitaCell Ltd in the United States and other countries. ValitaCell is a Beckman Coulter Company.