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细胞系开发的整体解决方案
表达重组靶点(如单克隆抗体、孔通道和 GPCRs)的稳定和瞬转细胞系常用于药物筛选、基因功能研究、细胞系筛选和安全评估研究。细胞系的开发过程从将感兴趣的质粒载体转染选定的宿主细胞(通常是 CHO 或 HEK 293 细胞)开始,然后根据细胞功能、产量和/或稳定性进行筛选和压力选择。选择后,根据它们的应用,细胞系和/或细胞产生的蛋白质被验证有效。由于手动筛选方法耗时、耗力且容易出错,因此对高通量、自动化筛选解决方案的需求很大。
贝克曼库尔特的 Biomek 工作站提供一套全方位的高通量自动化平台,可轻松整合,以满足细胞系开发工作流的每个关键步骤。
- 稳定细胞系开发
- 瞬转细胞系开发