DNAdvance - 哺乳动物组织 DNA 提取试剂盒

DNAdvance - 哺乳动物组织 DNA 提取试剂盒

高通量基因组 DNA （gDNA） 分离试剂盒，能够从哺乳动物组织样本中纯化高质量的 DNA。

  • 与各种下游分析工具兼容（例如 PCR、qPCR、SNP 基因分型和 NGS）
  • 手动和自动化友好型试剂盒
  • 采用自动化在 ±1 小时内处理三个 96 孔板
Resources + Tools
Technical Documents Training Performance Liquid Handlers Support FAQs

Explore From Tissue Models

DNAdvance - 哺乳动物组织 DNA 提取试剂盒工作流程视图

Genomics DNAdvance Workflow

试剂盒特点

概述
DNAdvance 是一种高通量 gDNA 分离试剂盒，能够从哺乳动物组织样本中纯化高质量的 DNA。
 

快速周转和可扩展性
将 DNAdvance 试剂盒与 Biomek 工作站相结合，可提供高通量和无人值守的实验解决方案；96 个样品 15 分钟的手动操作时间和1小时46分钟的 96 孔板操作时间。
 

核酸的高回收率
DNAdvance 始终提供卓越的核酸回收能力。从各种组织类型（如大脑、肾脏、肝脏、肺和尾巴）分离出多达 40 微克的 DNA。
 

下游应用
采用 DNAdvance 分离的 DNA 与各种下游分析工具兼容，如 PCR、qPCR、SNP 基因分型和测序。
 

引文

Cain-Hom, C., Splinter, E., van Min, M., Simonis, M., van de Heijining, M., Martinez, M., . . . Warming, S. (2017, January 4). Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification. Nucleic Acids Research, 45(8), e62. doi:10.1093/nar/gkw1329

  • Used to extract DNA from mouse tissue

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V., Nguyen, N. T., Zheng, Z., . . . Joung, J. K. (2015, July 23). Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature, 523(7561), 481–485. doi:10.1038/nature14592

  • Used to extract DNA from U2OS.EGFP human cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample We are currently developing a product using proprietary bead based technology that selectively binds DNA to the beads to effectively separate DNA and RNA independently. This allows for the extraction of all nucleic acids from one experimental setup
SPRI Methodology Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.
QMJ_DNAdvance
Isolation of DNA from Buccal Swabs using DNAdvance Kit This protocol increases the volume of lysis buffer so that these two swabs from a single individual can be run in a single well and have the swab heads covered by lysis buffer.

技术文件

TechDocs.MoreResultsMessage 此处。