绝对计数微球试剂盒

淋巴细胞亚群的细胞绝对数和百分比值通常使用自动血球计数仪器上的白细胞数和淋巴细胞数乘以流式细胞仪上测量的阳性染色细胞百分比。然而，这种标准（间接）方法却存在各个实验室间的变动差异。1-4 流式计数荧光微球是这种绝对计数标准（间接）方法的有效替代选择，通过单平台流式免疫表型分析直接提供绝对计数结果。当生物标本和流式计数荧光微球的相同体积存在时，将确定试样中细胞与荧光微球的比例，目标细胞和荧光微球随后通过流式细胞测定。

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AQUIOS Flow-Count Fluorospheres

 AQUIOS Flow-Count Fluorospheres is a fluorescent microsphere reagent for direct determination of leukocyte absolute counts in biological specimens on the AQUIOS CL flow cytometer. Flow-Count Fluorospheres are a suspension of fluorescent microbeads used to determine absolute counts on the flow cytometer. Each fluorosphere contains a dye which has a fluorescent emission range of 525 nm to 700 nm when excited at 488 nm. They have uniform size and fluorescence intensity, and an assayed concentration allowing a direct determination of absolute counts.

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