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Valita Titer Assay — A Simplified Workflow
The Valita Titer assay is one of the fastest and easiest IgG quantification assays because of its simple add-mix-read workflow.
In just three steps, and in as little as 15 minutes, you’ll be able to prepare and measure a full 96- or 384-well plate. There’s no sample prep, additional reagents or wash required, so even if you wanted to measure non-purified samples directly from a bioreactor, you could do so with ease.
Add fresh media/IgG sample and mix
Incubate for five minutes at room temperature
Measure IgG using a plate reader equipped with a fluorescence polarization module
About the Valita Titer Assay Principle
The Valita Titer assay uses fluorescence polarization (FP) for detection. FP effectively analyzes changes in the size of molecules, given that smaller molecules tumble more rapidly than larger ones in solution.
Valita Titer plates come pre-coated with an IgG Fc-specific fluorescently labeled probe. The probe is reconstituted using fresh cell culture media. So, to measure IgG effectively and rapidly in your lab, all you’d need is a fluorescence polarization-enabled microplate reader and filters that are compatible with green fluorescent dyes such as fluorescein.
Insightful Resources
Application Note
High-throughput IgG quantitation platform for clone screening during drug discovery and development.
High-throughput IgG quantitation platform for clone screening during drug discovery and development.
Application Note
Increased throughput for IgG quantification using Valita Titer 384-well plates
Increased throughput for IgG quantification using Valita Titer 384-well plates
Application Note
Automating the Valita Titer IgG Quantification Assay on a Biomek i-Series Liquid Handling System
Automating the Valita Titer IgG Quantification Assay on a Biomek i-Series Liquid Handling System
Technical Note
Rapid Rabbit IgG Quantification using the Valita Titer Assay
Rapid Rabbit IgG Quantification using the Valita Titer Assay
Learn More About the Valita Titer Assay
Introducing Valita Titer Assays
Valita Titer plate-based, 96- and 384-well assays that offer a rapid, cost-effective way to measure IgG
Compare IgG Measurement Technologies
See how the Valita Titer assay compares to Protein A HPLC, ELISA and biolayer interferometry
Automate With Ease
Easily integrate Valita Titer into your automated liquid handler and microplate reader
Cell Line Development Webinar
Speed up your clone selection process with the Valita Titer assay
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