DNA Extraction from Tissue

DNAdvance

A high-throughput genomic DNA (gDNA) extraction reagent kit that enables purification of high-quality DNA from mammalian tissue samples.

  • Compatible with a variety of downstream analysis tools (e.g., PCR, qPCR, SNP genotyping and NGS)
  • Manual and automation-friendly chemistry
  • Process three 96-well plates in ~1 hour using automation
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DNAdvance - 哺乳动物组织 DNA 提取试剂盒

Explore From Tissue Models

DNAdvance for Tissue Workflow

Genomics DNAdvance Workflow

Product Features

Overview
DNAdvance is a high throughput gDNA isolation reagent kit that enables the purification of high quality DNA from mammalian tissue samples.

Turnaround and Scale
Combining the DNAdvance kit with Biomek Workstations offers a high throughput and walk-away solution; 15 mins hands-on-time for 96 samples and 1 hr 46 mins for 96 well plate.

High Recovery of Nucleic Acids
DNAdvance consistently delivers superior recovery of nucleic acids. Isolate up to 40 ug of DNA from a variety of tissue types, e.g. brain, kidney, liver, lung, and tail.

Downstream Applications
DNA isolated with DNAdvance is compatible with a variety of downstream analysis tools such as PCR, qPCR, SNP genotyping, and sequencing.

Citations

Cain-Hom, C., Splinter, E., van Min, M., Simonis, M., van de Heijining, M., Martinez, M., . . . Warming, S. (2017, January 4). Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification. Nucleic Acids Research, 45(8), e62. doi:10.1093/nar/gkw1329

  • Used to extract DNA from mouse tissue

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V., Nguyen, N. T., Zheng, Z., . . . Joung, J. K. (2015, July 23). Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature, 523(7561), 481–485. doi:10.1038/nature14592

  • Used to extract DNA from U2OS.EGFP human cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

QMJ_DNAdvance
Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample We are currently developing a product using proprietary bead based technology that selectively binds DNA to the beads to effectively separate DNA and RNA independently. This allows for the extraction of all nucleic acids from one experimental setup
Isolation of DNA from Buccal Swabs using DNAdvance Kit This protocol increases the volume of lysis buffer so that these two swabs from a single individual can be run in a single well and have the swab heads covered by lysis buffer.
Simultaneous DNA & RNA Extraction from Cultured Cells without Splitting Lysate We developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same cultured cell sample. The method utilizes a proprietary buffer to selectively bind RNA and DNA
Simultaneous DNA and RNA Extraction from Tissue without Splitting Lysate In this protocol we developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same fresh/frozen sample. The method utilizes size selection to separate DNA and RNA

Technical Documents

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