OptiMATE Gradient Maker for Centrifugation
Automated Density Gradient Sample Preparation
The OptiMATE Gradient Maker simplifies the laborious and error-prone density gradient centrifugation process into an easy-to-maintain operation by automating nearly all pre-centrifuge steps, including gradient preparation, accurate dispensing, and tube sealing, significantly reducing run times without sacrificing purity or yield. The OptiMATE Gradient Maker has achieved the ACT label in the US, EU, and UK.
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Explore OptiMATE Gradient Maker Models
Features of the OptiMATE Gradient Maker
Automated dispense and tube sealing
- Significantly reduce error and improve reproducibility
- Compatible with most common Beckman Coulter tube sizes
- Compatible with Quick-Seal, OptiSeal, and Open-Top tubes
Pre-formed gradients
- Ability to dispense linear and step gradients
- Dramatically shorter run times (75+% reduction) for linear gradients
Simple GUI and method creation
- Train new operators in just hours, not weeks
- Published methods ensure consistency between runs and operators
- On-board visualization of gradient profiles
Automated dispense and tube sealing
Pre-formed gradients
Simple GUI and method creation
- Automated dispensing of both step and linear gradients, and automated tube sealing
- Significantly shorter run times by starting with pre-formed linear gradients
- Improved consistency – reduce errors with automation
- Generate high-quality gradients in just hours even without density gradient proficiency
- Compatible with cesium chloride (CsCl), iodixanol (IDX), and sucrose
Although gradient profiles indicate sufficient separation even at 4 hours, control pre-formed experiments (left) were run for 5 hours while AAV pre-formed experiments (right) were run for 5 hours to improve band resolution/sharpness.
Resources
Reducing Variability and Hands-On time in Viral Vector purification using the OptiMATE Gradient Maker
OptiMATE Gradient Maker
Features Overview
The OptiMATE Gradient Maker has achieved the ACT label – a sustainability certification for lab products focused on Accountability, Consistency, and Transparency – in the US, EU, and UK.
Accessories
Density Gradient Reagents
OptiMATE Iodixanol Solution
Iodixanol for your purification needs. Ideal for step gradients common to viral, organelle, EV, and protein separations. Concentrated to 80%, OptiMATE Iodixanol can enable you to increase your sample per tube and decrease your bottle-related waste.
Volume: 500mL, Density: 1.420 ± 0.004 g/mL, Concentration: 80% w/v, Refractive Index: 1.460 ± 0.004, Endotoxin Level: ≤1 EU/mL, Sterile: Yes (autoclaved), Shelf Life: 18 months, Bottle Dimensions: 17.6 cm x 7.2 cm / 6.93" x 2.83" (H x W)
OptiMATE Cesium Chloride Solution
Highly concentrated, premixed CsCl solution offering significant time savings in reagent preparation. Ideal for continuous gradients requiring the highest quality purification, commonly used in viral, plasmid, or nucleic acid purification.
Volume: 500mL, Density: 1.784 ± 0.014 g/mL, Concentration: 60% w/w*, Refractive Index: 1.408 ± 0.004, Endotoxin Level: ≤1 EU/mL, Sterile: Yes (autoclaved), Shelf Life: 18 months, Bottle Dimensions: 17.6cm x 7.2 cm / 6.93" x 2.83" (H x W)
Frequently Asked Questions
- Cesium Chloride
- Iodixanol (OptiPrep™)
- Sucrose
- 16 mm (8.9 to 13.5 mL)
- 25 mm (29.9 to 39 mL)
- 38 mm (94 mL)
- Quick-Seal
- OptiSeal
- Open-Top
- Polypropylene
- Ultra-Clear
- Viruses / viral vectors (e.g., AAV)
- Virus-like particles and bacteriophage
- Extracellular vesicles (EVs)
- Proteins / protein complexes
- Lipid nanoparticles
- Synthetic nanoparticles or other nanomaterials
- Nucleic acids / plasmids
- Lipemic separations
