Innovative LNP Formulation and Analysis Solutions

The increasing demand for novel lipid nanoparticle formulations is driving the need for high-throughput development and screening workflows. We offer comprehensive solutions for producing and purifying nucleic acids for LNP loading, LNP mixing, drug formulation, and analytical characterization.

Lipid nanoparticles (LNPs) are a promising drug delivery system composed of a mix of various lipids (e.g., helper, ionizable, PEGylated, targeting) and often cholesterol with an encapsulated cargo. We offer a workflow solution to optimize LNP formulations, utilizing automation and ultracentrifugation, as well as solutions of biophysical and function studies with analytical ultracentrifugation and flow cytometry.

LNP Workflow

Drug Development

Therapeutics, including small molecules (e.g., antibiotics, chemotherapy agents), biologics (e.g., antibodies, hormones), and nucleic acids (e.g., mRNA, siRNA), are prepared using ready-to-use reagents, liquid handlers, automated cell culture workstations, centrifuges, and chromatography systems.

Biomek i-Series Automated Workstation

Echo 650 Series Acoustic Liquid Handlers

Lipid Nanoparticle Production

LNP formulations incorporating novel ionizable cationic lipids are at the forefront of RNA delivery technologies. Encapsulation involves using a microfluidic mixer to facilitate the controlled nanoprecipitation of lipids and mRNA in an acidic buffer through electrostatic interaction.

Biomek i-Series Automated Workstation

Echo 650 Series Acoustic Liquid Handlers

Optima X Series Ultracentrifuges

Final Preparations

Following encapsulation, buffer exchange is performed to facilitate the rearrangement and stabilization of the nanoparticles. Fractionation of LNPs with different loading levels is also critical and can be achieved with centrifugation to ensure optimal transfection potency.

Biomek i-Series Automated Workstation

Optima MAX Series Benchtop Ultracentrifuges

Optima X Series Ultracentrifuges

Analysis & Characterization

Characterizing LNP attributes, such as LNP size, encapsulation efficiency, drug purity, heterogeneity, and mRNA/siRNA loading levels, before in vitro or in vivo use is essential for reproducibility and can be achieved with the Optima AUC Analytical Ultracentrifuge, LS 13 320 XR Particle Size Analyzer, and CytoFLEX Flow Cytometers.

Optima X Series Ultracentrifuges

CytoFLEX nano Flow Cytometer

CytoFLEX Platform

LS 13 320 XR Particle Size Analyzer

Our Solutions

With our expertise and advanced technology, we are equipped to support your research and development efforts efficiently and effectively.

Optimization and Purification of LNPs

Utilizing the Optima XPN Ultracentrifuge can help optimize the mRNA loading levels in formulations through fractionation.

Quantification and Characterization of LNPs

Utilizing the CytoFLEX nano Flow Cytometer, you can assess the payload of LNPs. The Optima AUC Ultracentrifuge enables the precise quantification of LNP loading states, hydrodynamic radius, and RNA copy number distribution.