Protein Production

Protein ProductionAmong the several methods currently available for protein purification, only one offers the benefits of a largely matrix-free system, allowing proteins to be purified in their native state. This is important because protein conformation and behavior can be altered due to interactions with membranes or beads, changing the true character of the protein you seek to characterize. Under matrix-free conditions, such as those found within high-performance centrifuges, it becomes possible not only to purify by molecular weight, but also by density and conformation, features unavailable with membrane-based purification techniques. A centrifuge can generate the exact forces needed to purify (and, when needed, quantitate) a protein sample for research or therapeutic use.

Review the basics or polish your technique with our application resources below.

Products for Protein Production

Avanti JXN 26 high speed centrifuge

Avanti JXN-26 Series High Performance

• Features support GMP Compliance
• Quickly isolate cells
• Rapidly remove debris
• Safeguard your final product

Optima Analytical Ultracentrifuge

• Characterizes quality
• Consistently verifies purity
• More reliable data analysis
• Real-time data analysis
Optima XPN ultracentrifuge

Optima XPN Ultracentrifuge

• Features support GMP Compliance
• Purify high-quality plasmids for optimal transfection 
 Sterile + Certified Free Round Bottom Polypropylene centrifugation Tube box

Sterile + Certified Free Tubes

• Maximize consistency of a high-quality final product
• Optimize workflow
• Improve data quality
1L HarvestLine System Liners Kit

HarvestLine System Liners

• Reduce biologics exposure
• Minimize sample contamination risks
• Optimize workflows
carousel of the Vi-CELL BLU Cell Viability Analyzer

Vi-CELL BLU

• Optimize analysis parameters for improved accuracy and correlation to alternative methods
• 24-position auto sampler enables walkway operation

CytoFLEX LX

• Quantify or characterize cells with specific biomarkers

*Research Use Only. Not for use in diagnostic procedures.

Biomek 4000

• Precisely track liquid level of tubes
• Provide a distinct interface
• Consistent fraction every time

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Content and Resources

Centrifugation is a complete workflow solution for protein purification and protein aggregation quantification Protein aggregation is a biological phenomenon caused by the abnormal interaction of destabilized proteins. Characterization of protein behavior in solution is critical, as destabilized proteins are susceptible to chemical and physical alterations leading to loss of function.
g-Max: Added Capabilities to Beckman Coulter's versatile Ultracentrifuge Line The g-Max System adds several valuable capabilities to your Beckman Coulter ultracentrifuge rotors:
Linearity of BSA Using Absorbance & Interference Optics The Optima AUC is the latest innovation from Beckman Coulter and offers significant advantages as an analytical ultracentrifugation platform over the predecessor ProteomeLab. Besides the plethora of advancements in user experience on the new platform, the Optima AUC’s optical, thermal, and drive systems have also been upgraded significantly, creating an overall better experience to the end-user in terms of data quality and ease of use.
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation Insulin is a well characterized drug used to treat diabetes. A large number of insulin formulations have been investigated in order to develop the optimum time delivery suited to patient needs. In addition, a number of insulin analogs such as Insulin Glargine and LysPro insulin (Humalog) have been developed and marketed as derivatives for immediate use at meal time and a more sustained release.
Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin <p style="margin-right: 0px; margin-bottom: 0px; margin-left: 0px;">Analytical Ultracentrifugation (AUC) is the method of choice to characterize colloidal systems under physiological solution conditions.</p> <p style="margin-right: 0px; margin-bottom: 0px; margin-left: 0px;">Recently, a novel multi-wavelength (MWL) detector on Beckman Coulter&rsquo;s Optima AUC has been developed, which for the first time, couples the hydrodynamic separation of colloidal mixtures to spectral deconvolution of interacting and non-interacting solutes present in a mixture.</p>