PerFix-nc Cell Permeabilization Kit, RUO, 75 Tests
Product No:B10825
The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation.
Accurate detection of both intracellular and extracellular epitopes are obtained, while:
• There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional).
• Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step.
• Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
• Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.
Accurate detection of both intracellular and extracellular epitopes are obtained, while:
• There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional).
• Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step.
• Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
• Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.
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Product Specifications
| Fluorochrome | - |
| Regulatory Status | RUO |
| Size | 75 Tests |
| Flow Product Line | - |
| Kit Components | Fixative Buffer (2 vials/150 tests), Permeabilizing Buffer (2 vials/150 tests), Final 10X Solution (2 vials/150 tests) |
