Explore New Spectral Territory With Infrared Antibody Conjugates
As high-parameter flow cytometry is becoming mainstream, there is a growing demand for ”low-noise“ fluorochromes, i.e., dyes which are characterized by:
- Main excitation by a single laser line
- Negligible emission overlap with other dyes
- Low autofluorescence burden
- Moderate brightness
That‘s why we are expanding our dye portfolio outside the crowded visible spectrum with the new infrared fluorescent IR820 and IR870 dye antibody conjugates for flow cytometry. Move gating markers far away from where your most precious dim signals reside and get more out of your IR laser with IR820 and IR870 reagents!
Single Laser Line Excitation, Negligible Emission Overlap
In contrast to the vast majority of fluorescent dyes used in flow cytometry, infrared fluorochromes IR820 and IR870 are mainly excited by the infrared laser, hence emitting in the infrared range with practically no overlap with other fluorochrome emissions.
Ultralow Autofluorescent Background Is Inherent To IR Laser Excitation
Autofluorescence presents significant challenges in flow cytometry, particularly in spectral flow cytometry, complicating the detection, quantification and interpretation of fluorescence signals. IR820 and IR870 signals are nearly devoid of autofluorescence by design, as they are excited and fluoresce in the infrared range.
Infrared Fluorochromes IR820 and IR870 - Moderate Bright Signals, Suited For Gating Markers
IR820 and IR870 conjugates deliver signals which match the brightness needed for clear separation of gating makers, but their emissions are still moderately bright so they do not cause mutual spillover spreading.
Infrared Dye Conjugated Antibodies
Do you want to extend or design a new antibody panel with ”low-noise“ lineage markers?
We‘re happy to introduce IR820 and IR870 conjugates, which enable you to move important lineage markers into the infrared spectrum, eliminating spreading from bright gating antigens into the visible spectral range. Gain more flexibility in selecting bright fluorochromes for your critical co-expression markers of interest!
Explore our Infrared IR820 and IR870 dyes
IR820 and IR870 Antibody Conjugates - Compatible with a Broad Range of Dyes
No undesired dye-dye interactions could be observed for the CD20-IR820 conjugate when used within a 21-color panel. Data was acquired from stained and lysed whole blood on a CytoFLEX LX flow cytometer equipped with a CytoFLEX mosaic 88 spectral detection module.
IR820 Antibody Conjugates - Compatible With Fixable Viability Dye ViaKrome 808
CD45-IR820 could be used together with the infrared fixable viability dye ViaKrome 808 to clearly identify viable human leukocytes in the sample. Data was acquired from stained and lysed aged whole blood on a CytoFLEX LX.
IR820 and IR870 Antibody Conjugates - Drop-in Markers For The DURA Innovations Format
CD14-IR870 and CD20-IR820 were added to the 10-color DURAClone IM T Cell Subset dry antibody panel (B53328) without observing any interferences with the well-known staining patterns.
For Research Use Only. Not for use in diagnostic procedures.
Pacific Blue ("PB"), Alexa ("A”), and Alexa Fluor ("AF") are trademarks or registered trademarks of Thermo Fisher Scientific Inc. Brilliant Violet ("BV") and Brilliant Ultra Violet (“BUV”) are trademarks or registered trademarks of Becton, Dickinson and Company.
