Introducing the Next-Generation
Basophil Activation Test (BAT)

Introducing the Next-Generation
Basophil Activation Test (BAT)

Our new custom next-generation Basophil Activation Test (BAT) based on our award-winning solution can be provided through our Custom Design Service (CDS). You can choose the allergens and antibodies for your custom dry antibody panel. No antibody and no allergen pipetting needed!

Leveraging dry technology to overcome traditional BAT challenges

• Fostering standardization: Conjugated antibodies and allergens are pre-mixed at desired dose, and dried down together in a single tube. Negative and positive controls can be included.
• Straightforward: add your sample, vortex and acquire your data.

• Easy to use: no compensation, no centrifuge, compatible with any flow cytometer.
• Customizable: define your own combination of antibodies and allergens with specifications and format of your choice.

What is a Basophil Activation Test (BAT)?

BAT is a functional assay for measuring basophil activation in response to specific allergens or other stimuli.

Basophils play a critical role in allergies by releasing histamine and other mediators when activated. During a Basophil Activation Test a sample of blood is exposed to suspected allergens and then analyzed on a flow cytometer to identify the activation of basophils using conjugated antibodies specific to activation markers expressed on the surface of basophils.

However, due to the perceived complexity of the tools, BAT has not been widely adopted so far. Ready-to-use dry reagents can support driving adoption by being straightforward, standardized and easy to use, giving access to BAT insights even to non-expert laboratories.

BAT is minimally invasive and could potentially be complementary to traditional tools used in the study of allergies.

This new BAT enhances clinical trial safety & precision

Until now, the absence of a standardized BAT limited its potential use in clinical trials, thereby affecting drug safety assessments, patient stratification and regulatory compliance in the field of allergy treatment.

and regulatory compliance in the field of allergy treatment. Our a next-generation BAT ensures:

Customized to your study requirements

Ready-to-use, dry and shelf-stable BAT reagents are provided in pouches that can be shipped/stored at room temperature. Each pouch contains the tubes required for characterization of whole blood samples (heparinized or EDTA-anticoagulated). You can customize pouch contents to meet your study requirements, including:

• Pre-built allergen dose response curves
• Multiple allergenic compounds
• 1 or more concentrations
• Negative and positive controls

A smarter workflow with BAT

The same centrifugation-free protocol, consisting of only 4 pipetting steps, can then be implemented to prepare the tubes, resulting in a streamlined, robust and standardizable approach to BAT that can be used in most laboratories, regardless of flow cytometry expertise.

A promising outlook for BAT

Our next-generation BAT technology led to the solution being selected as the most promising approach to create an accurate, accessible, user-friendly test for the study of food allergies by FARE, the US leading non-profit organization engaged in food allergy advocacy.

Prepared BAT samples are stable for days

Published data* show that stained samples can remain stable at room temperature for up to 5 days. Even without flow cytometry expertise, labs can prepare samples on-site in dry DURAClone tubes and send them to a reference lab for analysis. This helps ensure reliable results without immediate processing or special storage conditions.

*A novel approach to the basophil activation test for characterizing peanut allergic patients in the clinical setting Agyemang A, Suprun M, Suarez-Farinas M, Boina F, et al., Allergy 2021, 76, 2257

Available allergen extracts

We’re partnering with InBio, a global leader in purified food allergens, to𠊎nhance the accuracy and consistency of our Next-Generation BAT. Alongside other major suppliers, this collaboration provides access to high-quality protein extracts for standardized allergen stimulation, improving test performance and offering a safer, more efficient alternative to oral food challenges.

For Research Use Only. Not for use in diagnostic procedures.

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